3D printed Multi-Walled Carbon Nanotube / Polylactic Acid Scaffolds to regenerate to dentin-pulp complex tissue It is very important to develop new-generation biomaterials for repairing dental structures and restoring their biological functions. One of the biggest obstacles to successful tissue regeneration is to regenerate tissue with appropriate structural properties. For example, in a natural tooth, dentin is a mineralized hard tissue composed of numerous contiguous dentinal tubules a few micrometers in diameter. This unique tubular structure plays a crucial role in maintaining the normal mechanical and biological functions of the tooth. By using a polymer and carbon nanotube-based tissue engineering strategy, it is possible to replace damaged tooth structures and provide regenerative, supportive artificial tissues. In this study, the biocompatibility of polylactic acid and the superior physicochemical properties of carbon nanotubes will be used to achieve dental pulp stem cell (DPSC) differentiation of scaffold stiffness and permeability.
With the special scaffold to be designed, a structure that can provide full regeneration in the pulp dentine complex will be optimized in terms of stiffness, permeability, and tubular design.
PLA is one of the biodegradable polymers that has attracted a lot of attention in recent years due to its wide range of uses in biomedical fields. Combining PLA with various materials is one of the most practical strategies for improving PLA performance. By this strategy, carbon nanotubes (CNTs) were preferred to improve PLA performance due to their superior physicochemical properties, robustness, biocompatibility and positive contribution to biointegration in the research to be conducted here. By using CNTs, the mechanical strength of the scaffold will be increased and dentinogenesis will be supported. The characterization of the scaffold structures obtained by mixing at different concentrations will be performed by calorimetric and spectroscopic methods. Thus, 3D-printed Multi-Walled Carbon Nanotube (MDNT) / Polylactic Acid scaffolds will be optimized according to the desired qualities and properties, being a potential scaffold for the regeneration of dentin-pulp complex tissue. It is anticipated that the included MDNTs can improve the mechanical strength, bioactivity, and cell adhesion of the nanofiber polylactic acid scaffold.
In cell culture studies to be carried out for this purpose, first, the effects of the scaffold on cell viability and its integration with cells will be examined by staining and microscopic methods. In addition, its effects on cell viability will also be examined. In addition, cell differentiation will be observed in cells isolated from primary teeth. In light of the data obtained, it is planned to conduct animal experiments with the optimized skeletal structure. This study aims to optimize 3D-printed Multi-Walled Carbon Nanotube (MDNT) / Polylactic Acid scaffolds with desired qualities and properties to be a potential scaffold for the regeneration of dentin-pulp complex tissue. It is anticipated that the included MDNTs can improve the mechanical strength, bioactivity, and cell adhesion of the nanofiber polylactic acid scaffold.
Araştırmacı TÜSEB
Aflatoksin tespitinde kullanılmak üzere yapay antikor oluşturulması ve biyosensör sistemlerinde kullanılması Araştırmacı ARAŞTIRMA PROJESİ
